The Evolution of Improved Diagnosis of Coronary and Vascular Disorders-Juniper Publishers
JUNIPER PUBLISHERS - OPEN ACCESS JOURNAL OF CARDIOLOGY & CARDIOVASCULAR THERAPY Opinion The diagnosis of myocardial infarction leaped forward when Arthur Karmen [ 1 ] devised the spectrophotometric method for measuring aspartame aminotransferase (AST) in serum in 1954. Prior to this, there was only the electrocardiogram and characteristic chest pain. In these studies, Karmen [ 1 ] demonstrated that acute myocardial infarct and also hepatic necrosis can be determined by measuring the serum elevation of either AST or on the other hand, alanine aminotransferase. He also measured the NADH linked lactate and malate dehydrogenases, of which Kaplan [ 2 ] found had enzymes, of which H-type LD was used for the diagnosis of myocardial infarct and the M-type for the diagnosis of hepatic or muscle injury. The H-type LD was elevated to a peak on the second day after infarct. But these tests were followed by creatine kinase (CK) and the CK-MB isoenzyme, which had an earlier ri...